Bioremediation of 2,4,6-trinitrotoluene by bacterial nitroreductase expressing transgenic aspen.
Identifieur interne : 003985 ( Main/Exploration ); précédent : 003984; suivant : 003986Bioremediation of 2,4,6-trinitrotoluene by bacterial nitroreductase expressing transgenic aspen.
Auteurs : Pieter Van Dillewijn [Espagne] ; José L. Couselo ; Elena Corredoira ; Antonio Delgado ; Rolf-Michael Wittich ; Antonio Ballester ; Juan L. RamosSource :
- Environmental science & technology [ 0013-936X ] ; 2008.
Descripteurs français
- KwdFr :
- 2,4,6-Trinitro-toluène (isolement et purification), 2,4,6-Trinitro-toluène (métabolisme), Absorption (MeSH), Adaptation physiologique (MeSH), Bactéries (enzymologie), Culture hydroponique (MeSH), Distribution tissulaire (MeSH), Dépollution biologique de l'environnement (MeSH), Isotopes du carbone (MeSH), Milieux de culture (MeSH), Nitroréductases (métabolisme), Polluants du sol (métabolisme), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Pousses de plante (métabolisme), Racines de plante (métabolisme), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Populus.
- enzymologie : Bactéries.
- génétique : Populus.
- isolement et purification : 2,4,6-Trinitro-toluène.
- métabolisme : 2,4,6-Trinitro-toluène, Nitroréductases, Polluants du sol, Populus, Pousses de plante, Racines de plante.
- Absorption, Adaptation physiologique, Culture hydroponique, Distribution tissulaire, Dépollution biologique de l'environnement, Isotopes du carbone, Milieux de culture, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Absorption (MeSH), Adaptation, Physiological (MeSH), Bacteria (enzymology), Biodegradation, Environmental (MeSH), Carbon Isotopes (MeSH), Culture Media (MeSH), Hydroponics (MeSH), Nitroreductases (metabolism), Plant Roots (metabolism), Plant Shoots (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (growth & development), Populus (metabolism), Soil Pollutants (metabolism), Tissue Distribution (MeSH), Trinitrotoluene (isolation & purification), Trinitrotoluene (metabolism).
- MESH :
- chemical , isolation & purification : Trinitrotoluene.
- chemical , metabolism : Nitroreductases, Soil Pollutants, Trinitrotoluene.
- chemical : Carbon Isotopes, Culture Media.
- enzymology : Bacteria.
- genetics : Populus.
- growth & development : Populus.
- metabolism : Plant Roots, Plant Shoots, Populus.
- Absorption, Adaptation, Physiological, Biodegradation, Environmental, Hydroponics, Plants, Genetically Modified, Tissue Distribution.
Abstract
Trees belonging to the genus Populus are often used for phytoremediation due to their deep root formation, fast growth and high transpiration rates. Here, we study the capacity of transgenic hybrid aspen (Populus tremula x tremuloides var. Etropole) which expresses the bacterial nitroreductase gene, pnrA, to tolerate and take-up greater amounts of the toxic and recalcitrant explosive, 2,4,6-trinitrotoluene (TNT) from contaminated waters and soil. Transgenic aspen tolerate up to 57 mg TNT/L in hydroponic media and more than 1000 mg TNT/ kg soil, whereas the parental aspen could not endure in hydroponic culture with more than 11 mg TNT/L or soil with more than 500 mg TNT/kg. Likewise, the phytotoxicological limit for transgenic plants to a constant concentration of TNT was 20 mg TNT/L while wild-type plants only tolerated 10 mg TNT/L. Transgenic plants also showed improved uptake of TNT over wild-type plants when the original TNT concentration was above 35 mg TNT/L in liquid media or 750 mg TNT/kg in soil. Assays with 13C-labeled TNT show rapid adsorption of TNT to the root surface followed by a slower entrance rate into the plant. Most of the 13C-carbon from the labeled TNT taken up bythe plant (> 95%) remains in the root with little translocation to the stem. Altogether, transgenic aspen expressing pnrA are highly interesting for phytoremediation applications on contaminated soil and underground aquifers.
DOI: 10.1021/es801231w
PubMed: 18939578
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Here, we study the capacity of transgenic hybrid aspen (Populus tremula x tremuloides var. Etropole) which expresses the bacterial nitroreductase gene, pnrA, to tolerate and take-up greater amounts of the toxic and recalcitrant explosive, 2,4,6-trinitrotoluene (TNT) from contaminated waters and soil. Transgenic aspen tolerate up to 57 mg TNT/L in hydroponic media and more than 1000 mg TNT/ kg soil, whereas the parental aspen could not endure in hydroponic culture with more than 11 mg TNT/L or soil with more than 500 mg TNT/kg. Likewise, the phytotoxicological limit for transgenic plants to a constant concentration of TNT was 20 mg TNT/L while wild-type plants only tolerated 10 mg TNT/L. Transgenic plants also showed improved uptake of TNT over wild-type plants when the original TNT concentration was above 35 mg TNT/L in liquid media or 750 mg TNT/kg in soil. Assays with 13C-labeled TNT show rapid adsorption of TNT to the root surface followed by a slower entrance rate into the plant. Most of the 13C-carbon from the labeled TNT taken up bythe plant (> 95%) remains in the root with little translocation to the stem. Altogether, transgenic aspen expressing pnrA are highly interesting for phytoremediation applications on contaminated soil and underground aquifers.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18939578</PMID><DateCompleted><Year>2009</Year><Month>01</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0013-936X</ISSN><JournalIssue CitedMedium="Print"><Volume>42</Volume><Issue>19</Issue><PubDate><Year>2008</Year><Month>Oct</Month><Day>01</Day></PubDate></JournalIssue><Title>Environmental science & technology</Title><ISOAbbreviation>Environ Sci Technol</ISOAbbreviation></Journal><ArticleTitle>Bioremediation of 2,4,6-trinitrotoluene by bacterial nitroreductase expressing transgenic aspen.</ArticleTitle><Pagination><MedlinePgn>7405-10</MedlinePgn></Pagination><Abstract><AbstractText>Trees belonging to the genus Populus are often used for phytoremediation due to their deep root formation, fast growth and high transpiration rates. Here, we study the capacity of transgenic hybrid aspen (Populus tremula x tremuloides var. Etropole) which expresses the bacterial nitroreductase gene, pnrA, to tolerate and take-up greater amounts of the toxic and recalcitrant explosive, 2,4,6-trinitrotoluene (TNT) from contaminated waters and soil. Transgenic aspen tolerate up to 57 mg TNT/L in hydroponic media and more than 1000 mg TNT/ kg soil, whereas the parental aspen could not endure in hydroponic culture with more than 11 mg TNT/L or soil with more than 500 mg TNT/kg. Likewise, the phytotoxicological limit for transgenic plants to a constant concentration of TNT was 20 mg TNT/L while wild-type plants only tolerated 10 mg TNT/L. Transgenic plants also showed improved uptake of TNT over wild-type plants when the original TNT concentration was above 35 mg TNT/L in liquid media or 750 mg TNT/kg in soil. Assays with 13C-labeled TNT show rapid adsorption of TNT to the root surface followed by a slower entrance rate into the plant. Most of the 13C-carbon from the labeled TNT taken up bythe plant (> 95%) remains in the root with little translocation to the stem. Altogether, transgenic aspen expressing pnrA are highly interesting for phytoremediation applications on contaminated soil and underground aquifers.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Van Dillewijn</LastName><ForeName>Pieter</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Estacioín Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Department of Environmental Protection, Granada, Spain</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Couselo</LastName><ForeName>José L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Corredoira</LastName><ForeName>Elena</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Delgado</LastName><ForeName>Antonio</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Wittich</LastName><ForeName>Rolf-Michael</ForeName><Initials>RM</Initials></Author><Author ValidYN="Y"><LastName>Ballester</LastName><ForeName>Antonio</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Ramos</LastName><ForeName>Juan L</ForeName><Initials>JL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Environ Sci Technol</MedlineTA><NlmUniqueID>0213155</NlmUniqueID><ISSNLinking>0013-936X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002247">Carbon Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012989">Soil Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>118-96-7</RegistryNumber><NameOfSubstance UI="D014303">Trinitrotoluene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.7.-</RegistryNumber><NameOfSubstance UI="D009601">Nitroreductases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000042" MajorTopicYN="N">Absorption</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000222" MajorTopicYN="N">Adaptation, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002247" MajorTopicYN="N">Carbon Isotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018527" MajorTopicYN="N">Hydroponics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009601" MajorTopicYN="N">Nitroreductases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012989" MajorTopicYN="N">Soil Pollutants</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014018" MajorTopicYN="N">Tissue Distribution</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014303" MajorTopicYN="N">Trinitrotoluene</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>10</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>1</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18939578</ArticleId><ArticleId IdType="doi">10.1021/es801231w</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Ballester, Antonio" sort="Ballester, Antonio" uniqKey="Ballester A" first="Antonio" last="Ballester">Antonio Ballester</name><name sortKey="Corredoira, Elena" sort="Corredoira, Elena" uniqKey="Corredoira E" first="Elena" last="Corredoira">Elena Corredoira</name><name sortKey="Couselo, Jose L" sort="Couselo, Jose L" uniqKey="Couselo J" first="José L" last="Couselo">José L. Couselo</name><name sortKey="Delgado, Antonio" sort="Delgado, Antonio" uniqKey="Delgado A" first="Antonio" last="Delgado">Antonio Delgado</name><name sortKey="Ramos, Juan L" sort="Ramos, Juan L" uniqKey="Ramos J" first="Juan L" last="Ramos">Juan L. Ramos</name><name sortKey="Wittich, Rolf Michael" sort="Wittich, Rolf Michael" uniqKey="Wittich R" first="Rolf-Michael" last="Wittich">Rolf-Michael Wittich</name></noCountry><country name="Espagne"><noRegion><name sortKey="Van Dillewijn, Pieter" sort="Van Dillewijn, Pieter" uniqKey="Van Dillewijn P" first="Pieter" last="Van Dillewijn">Pieter Van Dillewijn</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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